Raw data of DAP-Seq of GiMYB76
收藏科学数据银行2025-09-25 更新2026-04-23 收录
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Genomic DNA from G. inflata was extracted by using phenol/ chloroform method, and purified DNA was quantified using a Qubit (Thermo Fisher Scientific). A total of 5 μg genomic DNA was sheared to ~200 bp fragments; the repaired ends were ligated Illumina-based sequencing adaptors to construct an adaptor-ligated DNA library.The ORF sequence of GiMYB76 was fused with the Halo affinity tag and expressed in vitro using TNT® SP6 Coupled Wheat Germ Extract System (Promega, Fitchburg, WI, USA). The fusion protein was bound to magnetic Halo Tag ligand (chloroalkane) beads, isolated from the expression mixture, and verified by Western blotting with anti-Halo Tag antibody (Promega), with nonspecific proteins were washed away. HaloTag-GiMYB76 fusion proteins were incubated with 500 ng of adaptor-ligated genomic DNA library at 30 ℃ for 2 h. Unbound DNA fragments were removed by washing, and samples were heated at 98 ℃ for 10 min to release GiMYB76 bound DNA The recovered DNA was amplified by PCR using indexed TruSeq primers. Indexed DNA samples were subsequently pooled and size-selected to remove residual adaptor dimers. Purified DNA libraries were subjected to next generation sequencing (PE150).The DAP-Seq data used in this study are also available from the National Genomics Data Center (https://ngdc.cncb.ac.cn/; PRJCA039808).
提供机构:
Zhigeng Wu; State Key Laboratory of Plant Diversity and Specialty Crops, Guangdong Provincial Key Laboratory of Applied Botany, Guangdong Provincial Key Laboratory of Digital Botanical Garden, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, Guangdong, China; University of Chinese Academy of Sciences, Beijing 100049, China; College of Life Science, Gannan Normal University, Ganzhou341000, Jiangxi, China; Yongqing Li; Yongliang Liu; Jixiang Zhu; Department of Plant and Soil Sciences, University of Kentucky, Lexington, KY 40546, USA; Jiangyi Zeng; Yuping Li; Jihua Wang
创建时间:
2025-09-25



