HuBMAP: Single-Cell Data from Human Tissues

The small bowel and colon are organs critical for maintaining homeostasis of the human body by mediating nutritional absorption upon the ingestion of food. Though both organs are extensive in length, there are known differences in function and cellular heterogeneity within different portions of each. Also, a cross section anywhere in the bowel reveals a complex layering of components involved in absorption and secretion, motility of gut contents, circulation, and immunity. We are mapping the complexity of the the small bowel and colon with cell-to-cell resolution in histologic sections, both along their lengths and across multiple individuals. Our molecular data consist of single cell ATAC-seq and RNA-seq. These profiles are spatially mapped to histologic sections using CO-Detection by indEXing (CODEX), a highly multiplexed system of antibody-tagged target epitopes.]]>
Inclusion: Organ donors from which consent can be obtained from next-of-kin for research activities and data/tissue distribution Cross-sectional imaging does not show evidence of bowel damage, such as malperfusion or pneumatosis Exclusion: Reactivity to HIV/HCV/HBV NAT, HBV surface antigen, HCV encoded antigen tests ]]>