Prevalence of virulence factor genes in major African and French Clonal Complexes causing meningitis.

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a Genotypic analysis performed using the following parameters: clonal complexes (CC) defined as MLVA types having a maximum distance of changes at 2 loci and a minimum cluster size of 2 types
b PMEN clones listed in www.sph.emory.edu/PMEN/.
cpspA not typable in 3 African and 3 French isolates, both families present in 1 French isolate, no family 3 identified
dnanA and nanB not reported because present in 100% of the strain collection
e Presence of a truncated gene with lower PM amplified product in 6 French isolates
Abbreviations: MLVA = Multi Locus Variable number tandem repeats Analysis, Sp = serotype, CC = Clonal Complex, CSF = cerebrospinal fluid, N = number, PMEN = pneumococcal molecular epidemiological network; ply coding for Pneumolysin, pspA for Pneumococcal surface protein A (all types+ family 1/2), pspC for Pneumococcal surface protein C (all types and group 4), pavA for Pneumococcal adhesion and virulence A, lytA for Autolysin A, phtA, B, D, E for Polyhistidine triad complex A, B, D, E, nanA, B, C for Neuraminidase A, B, C, rrgA for detection of Pilus-1 islet, sipA for detection of Pilus2 islet, pcpA for Pneumococcal choline binding protein A, psrp for Pneumococcal serine-rich protein.
Prevalence of virulence factor genes in major African and French Clonal Complexes causing meningitis.