Data and calculations used for estimating relative triploid vs diploid hybrid transcriptome size.
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1-Expression quantified by qRT-PCR, using total nucleic acid as the template for reverse transcription and normalization to genome copy number.
2- Because PAA has 3 genomes per cell, meaning 1,5x the amount of genomes per diploid cell, the transcripts/cell values for PAA/PA are equal to 1,5x the values for transcripts/genome.
3-For each target gene RPKM values were derived from the liver RNA-Seq data set.
4-Trascriptome size is determined by dividing “transcripts/cell” by “transcripts/transcriptome”.
Data and calculations used for estimating relative triploid vs diploid hybrid transcriptome size.
创建时间:
2015-03-19



