Danio rerio amh mutant RNA-Seq

For 21dpf and 35dpf juvenile zebrafish, trunk tissue was isolated by removing the anterior of the fish from just posterior of the pectoral fin and removing the caudal peduncle posterior to the anus. For adults, ovary and testis was dissected separately from other tissues. Total RNA was extracted using the Ribopure Kit (Thermo Fisher), and polyA mRNA was enriched using Dynabeads (Thermo Fisher). We constructed indexed, strand-specific cDNA sequencing libraries using the NEXTflex™ qRNA-seq kit (BIOO Scientific). Libraries were normalized to 2.3nM, multiplexed, and evaluated by quantitative real-time PCR using the Kapa Library Quantification Kit (Kapa Biosystems). One lane of paired-end 100 base pair (bp) sequencing was performed on an Illumina HiSeq 4000.