Naphthoquinone preferentially pairs with non-proton-pumping NADH dehydrogenase for respiratory electron transport

Naphthoquinones preferentially pair with the non-proton-pumping NADH dehydrogenase, thereby optimizing electron transport in low-potential respiratory chains under aerobic conditions. Growth of Isochorismate synthase and Chorismate pyruvate-lyase deficient E. coli MG1655 was performed in their respective evolution media, i.e. minimal media supplemented with glucose (4g/L) as the carbon source. The strains were grown at 37C in two biological replicates for each strain. Total RNA was extracted from the mid-log phase culture for RNA-seq library preparation.