Experimental evolution of biofilm hyperproduction in Pseudomonas aeruginosa

In this work, we leveraged an experimental evolution model originally described by Poltak and Cooper (2011) to adapt lineages of P. aeruginosa grown as biofilms on bead substrata of glass, PVC and type-319 stainless steel through serial passage. Genome sequencing of the experimentally evolved lineages was performed at across the passage series and the evolutionary trajectories associated with biofilm hyperproduction on each substratum material were characterised.