Triticum aestivum Transcriptome or Gene expression. Triticum aestivum

We have employed whole genome microarray expression profiling to identify genes conferring induction of pistillody, homeotic transformation of stamens into pistil-like structures. As a result, we identified five genes which show higher expression levels in pistillody line compared with normal line. Quantitative expression analysis using real-time PCR indicated that among five genes a calmodulin (CaM)-binding protein gene, WCBP1 (wheat calmodulin-binding protein 1), is obviously up-regulated in the young spikes of the pistillody line. The full-length cDNA sequence for WCBP1 showed it is a member of the ACBP60 family CaM-binding protein. Overall design: Expression patterns were compared between the pistillody line and normal line. Total RNA samples were isolated from young spikes (3-10mm in length) at floret differentiation stage. Two independent experiments were conducted in each experiments.