Epigenetic landscape of HEK cells upon epithelial-to-mesenchymal transition

Epithelial-to-mesenchymal transition (EMT) allows progressive loss of epithelial traits and acquisition of mesenchymal identity promoting cell migratory and invasive properties. It is expressed by changes in gene expression program induced by changes of chromatin state and organization. To identify epigenetic landscape associated with EMT we performed chromatin immunoprecipitation (ChIP) experiments allowing nucleosome profiling (H3), distribution of histone modifications linked to actively transcribed (H3K4me3, H3K36me3 and H3K27ac) and repressed (H3K27me3) regions in epithelial (Epi) and mesenchymal (Mes) cells. These cell lines were generated by immortalization of primary HEK cells before and after naturally occured EMT. Overall design: For the two Epi and Mes cell lines, crosslinked chromatin was purified from two independent biological duplicate cell cultures. For each replicate, an aliquot was kept as Input while the rest was used for Chromatin Immunoprecipitation using antibodies against H3, H3K36me3, H3K27me3, H3K27ac and H3K4me3.