Whole genome sequencing indicates <i>PMR1</i> linkage to NaCl resistance.

The indicated evolved clones obtained from Transfer 10 were each mated to an unevolved compatible strain (EAY3241 for mating to incompatible evolved, EAY3191 for mating to compatible evolved). The resulting diploids were sporulated and tetrad dissected and germinated spore clones were analyzed for growth on YPD containing 1.2 M NaCl. For each mating at least 18 NaClr and 18 NaCls spore clones were separately pooled. The resistant and sensitive pools were then analyzed by whole genome sequencing (Materials and Methods). The sequence for the indicated position is shown for the unevolved reference (WT) and the evolved strains (SNP), followed by the number of WT and SNP reads detected in each pool. Using the Fisher Exact test, all SNPs we found that were defined as linkage differ from random segregation with a p value <0.0001. Whole genome sequencing indicates PMR1 linkage to NaCl resistance.