Mesenchymal stromal cells-derived small extracellular vesicles affect T-lymphocytes mRNA expression

In the current research, we further study the immune-modulatory effect of bmMSCs on T cells, by mRNA sequencing of activated T cells co-cultured with bmMSC-sEVs. Methods: activated T lymphocytes were cocultured with and without bmMSC derived sEVs. mRNA profiling was performed using the QIAGEN Illumina sequencing platform. IPA® software, KEGG pathway, GSEA and STRING database were applied to analyze pathway networks and biological functions of the differentially expressed genes. Three batches of sEVs were isolated from three different bmMSC donor. Activated lymphocytes were isolated from one sample of buffy coat. The study evaluates acticated lymphocytes versus activated lymphocytes cocultured with three different batches of sEVs