eDNA metabarcoding of communities under agricultural stress. lake water metagenome

This study investigated the impacts of two agricultural stressors (the neonicotinoid insecticide thiacloprid and fertilizer) in full-factorial design in a semi-natural research site, using environmental DNA sampling to study three different taxonomic groups representing three trophic levels: bacteria (decomposers), phytoplankton (primary producers), and chironomids (consumers). eDNA samples of 20 artificial ditches were obtained at different time points prior and after application of stressors.