An efflux pump family distributed across plant commensal bacteria conditions host- and organ-specific detoxification of a host-specific glucosinolate

In this study we deployed barcoded bacterial mutant libraries (BarSeq) to characterized bacterial genes that benefit the association with plants. Mutant libraries of two commensal bacteria Paraburkholderia bryophila MF376 and Pseudomonas simiae WCS417 were harvested from either roots or shoots of two distinct host plants: Arabidopsis thaliana Col-0 and Brachypodium distachyon BD21. After enrichment of the bacteria, the barcodes of all bacterial population were sequenced and submitted to the archive.