A distinct pre-treatment immune gene signature in lentigo maligna is associated with imiquimod response

Raw Nanostring Files. Total RNA was extracted from FFPE tissue sections adjacent to sections stained by multiplex IHC, using the RNeasy® FFPE Kit (Cat. 73504, QIAGEN GmbH, Hilden, Germany). 10x10um thick sections were used per sample due to the small specimen size of each biopsy. Concentration was determined using Nanodrop™ 8000 Spectrophotometer (Thermofisher Scientific, PA) and 400ng of total RNA run on the nCounter analysis system™ (nanoString™, WA) using the PanCancer Immune Profiling Panel (nanoString™, WA) consisting of 770 genes. Files include: - Raw log2 expression matrix - Sample identifiers - Raw .RCC files - RLF file原始Nanostring文件。本研究从经多重免疫组化（multiplex IHC）染色的相邻福尔马林固定石蜡包埋（FFPE）组织切片中提取总RNA，采用RNeasy® FFPE试剂盒（货号73504，德国希尔登凯杰有限公司（QIAGEN GmbH））完成提取。因每份活检标本体积较小，每份样本使用10×10 μm厚度的切片。采用Nanodrop™ 8000分光光度计（赛默飞世尔科技，宾夕法尼亚州）测定RNA浓度，取400 ng总RNA，借助nCounter分析系统™（nanoString™公司，华盛顿州）结合包含770个基因的泛癌免疫分析探针组完成检测。所涵盖的文件如下： - 原始log₂表达矩阵 - 样本标识符 - 原始.RCC文件 - RLF文件