Transcriptomic analysis of Erwinia amylovora during colonization on trichomes of apple

In this study, we aimed to identify differentially expressed genes in the fire blight pathogen Erwinia amylovora during colonization on trichomes of apple. E. amylovora strain Ea110 was dip-inoculated onto apple leaves (Malus domestica, cultivar McIntosh) in an apple orchard. Glandular trichomes and non-glandular trichomes were collected from the inoculated leaves 3 days post inoculation. E. amylovora was collected from the trichomes through vortexing and sonicating, followed by the RNA isolation. Sequencing was performed on Illumina Novaseq. Differentially expressed genes were identified as compared to the original E. amylovora inoculum in phosphate buffer saline, from an overnight culture of E. amylovora in LB broth.

在本项研究中，我们旨在鉴定在苹果树毛细胞器上定殖期间，火疫病菌欧文氏菌（Erwinia amylovora）中差异表达的基因。选取了欧文氏菌菌株 Ea110，将其通过浸渍法接种于苹果叶片（Malus domestica，麦金托什品种）上，接种地点位于苹果园内。在接种后第3天，收集了腺毛和非腺毛细胞器。通过涡旋和超声处理从毛细胞器中收集欧文氏菌，随后进行RNA的提取。测序工作在Illumina Novaseq平台上进行。与磷酸盐缓冲盐溶液中的原始欧文氏菌接种物相比，确定了差异表达基因，这些基因来源于在LB肉汤中过夜培养的欧文氏菌。