Flow cytometry data for FACS sorting of lycopene-producing Saccharomyces cerevisiae
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This dataset contains flow cytometry data files for the detection and sorting of lycopene-producing Saccharomyces cerevisiae strains. Experimental design: - Control strain: lycopene-non-producing S. cerevisiae - Producer strain: metabolically engineered lycopene-overproducing S. cerevisiae - Instrument: MoFlo XDP flow cytometer (Beckman Coulter) - Excitation: 355 nm - Emission: 575 nm (FL7 channel) - Software: Summit v5.2 Files included: - FCS files for control strain, producer strain, and simulated mixed libraries (BS1: producer:control = 1:10; BS2: producer:control = 1:30) - Post-sort fractions: AS1 (low-threshold) and AS2 (high-threshold) Related publication: High-Throughput Flow Cytometric Detection and Sorting of Lycopene-Producing Saccharomyces cerevisiae Strains. For further information, please contact the corresponding author at tu_r@ctbu.edu.cn.



