Reversible and irreversible PAH in a rat model for MCT+Shunt-induced PAH and hemodynamic unloading.

PAH was induced by 60mg/kg MCT and an aorto-caval shunt. At different timepoints of PAH progression (day 14, 21 and 28 after MCT-injection), the left lung with PAH was hemodynamically unloading by unilateral orthotopic transplatation into a syngeneic, healthy recipient. All day 14 and 7/10 day 21 transplanted lungs showed reversal of PAH after LTx. All day 28 and 3/10 day 21 transplanted lungs showed PAH progression after LTx. Lung tissue of Reversible and Irreversible PAH and normal controls, acquired at LTx, was compared using RNA-seq. Reversible PAH: N=11. RNA extracted in 3 biological replicates. Irreversible PAH: N=7, RNA extracted in 3 biological replicates. Normal control: N=5, RNA extracted in 3 biological replicates.