Population genetics dataset for Antarctic krill (Euphausia superba): Restriction site-associated DNA sequencing (RAD-seq) and mtDNA sequencing
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This restriction site associated DNA sequencing (RAD-seq) dataset for Antarctic krill (Euphausia superba) includes raw sequence data and summaries for 148 krill from 5 Southern Ocean sites. A detailed README.pdf file is provided to describe components of the dataset. DNA library preparation was carried out in two separate batches by Floragenex (Eugene, Oregon, USA). RAD fragment libraries (SbfI) were sequenced on an Illumina HiSeq 2000 using single-end 100 bp chemistry. As there is no reference genome for Antarctic krill, a set of unique 90 bp sequences (RAD tags) was assembled from 17.3 million single-end reads from an individual krill. We obtained over a billion raw reads from the 148 krill in our study (a mean of 6.8 million reads per sample). The reference assembly contained 239,441 distinct RAD tags. The core genotype dataset exported for downstream data filtering included just those SNPs with genotype calls in at least 80% of the krill samples and contained 12,114 SNPs on 816 RAD tags.Sample collection table (comma separated):Southern Ocean Location, Sample Size, Austral Summer, Latitude, Longitude, IDEast Antarctica (Casey), 21, 2010/2011, 64S, 100E, CasEast Antarctica (Mawson), 22, 2011/2012. 66S, 70E, MawLazarev Sea, 38, 2004/2005 and 2007/2008, 66S, 0E, LazWestern Antarctic Peninsula, 16, 2010/2011, 69S, 76W, WAPRoss Sea, 23, 2012/2013, 68S, 178E, Ross
本数据集为南极磷虾（*Euphausia superba*）的限制性酶切位点相关DNA测序（restriction site associated DNA sequencing, RAD-seq）数据集，包含采自南大洋5个采样点的148只南极磷虾的原始测序数据与汇总统计信息。随数据集附带详细的README.pdf文件，用于说明数据集的各组成部分。 DNA文库制备由美国俄勒冈州尤金市的Floragenex分两批独立完成。研究采用SbfI限制性内切酶构建RAD片段文库，使用Illumina HiSeq 2000测序平台，以单端100 bp化学法完成测序。 由于南极磷虾暂无公开参考基因组，研究人员从单只磷虾的1730万条单端测序reads中组装得到一套独特的90 bp序列（RAD标签）。本研究的148只磷虾共获得超过10亿条原始测序reads，单样本平均测序量为680万条reads。该参考组装结果共包含239441条独特的RAD标签。 用于后续数据过滤的核心基因型数据集，仅保留了在至少80%的磷虾样本中成功完成基因型分型的单核苷酸多态性（single nucleotide polymorphism, SNP）位点，最终包含816个RAD标签上的12114个SNP位点。 样本采集表（逗号分隔格式）： 南大洋采样点,样本量,南半球夏季,纬度,经度,样本编号前缀 东南极洲（凯西站）,21,2010/2011,64°S,100°E,Cas 东南极洲（莫森站）,22,2011/2012,66°S,70°E,Maw 拉扎列夫海,38,2004/2005及2007/2008,66°S,0°E,Laz 西南极半岛,16,2010/2011,69°S,76°W,WAP 罗斯海,23,2012/2013,68°S,178°E,Ross
