Accurate prediction of RNA translation with a deep learning architecture

Validation of motifs around strong TTS. Known readthrough stop codon from VDR and their mutations (without change of amino acids) were tested for translation readthrough. No_stop: stop codon of VDR (TGA) was mutated to CGA; strong TTS1: stop codon of VDR was mutated to a strong TTS by changing the upstream 27nt and downstream 12nt sequences; strong TTS2: stop codon of VDR was mutated to a strong TTS by changing the upstream 27nt sequence; weak TTS: stop codon of VDR was mutated to a weak TTS by changing the upstream 27nt.针对强翻译终止位点（Translation Termination Site, TTS）周边基序的验证实验：本研究以源自维生素D受体（Vitamin D Receptor, VDR）的已知通读型终止密码子及其同义突变（不改变氨基酸序列）为测试对象，开展翻译通读活性检测。各组设置如下： No_stop组：将VDR的终止密码子（TGA）突变为CGA； strong TTS1组：通过改变VDR终止密码子上游27nt与下游12nt的序列，将其突变为强翻译终止位点； strong TTS2组：仅通过改变VDR终止密码子上游27nt的序列，将其突变为强翻译终止位点； weak TTS组：通过改变VDR终止密码子上游27nt的序列，将其突变为弱翻译终止位点。