Development of a cost-effective, multifunctional SNP panel and analysis workflow for wolf monitoring in Finland

Genetic monitoring is an essential tool for managing threatened or commercially valuable wildlife species. Ideally, genetic monitoring relies on straightforward protocols for genotyping and data handling, accommodates varying sample volumes, applies to non-invasive samples such as scat and hair, produces reliable and repeatable genotypes, and is cost-effective. Moreover, the marker panel itself should be tailored to specific species and/or population management information needs. We describe the development of a microarray-based 96-SNP panel specifically designed for non-invasive genetic monitoring of the Finnish wolf population. The panel was developed to meet four specific needs for this population: identification of wolf genotypes from the non-target species (fox, raccoon dog, golden jackal), species detection, sexing, individual identification, and kinship resolution. Detection of potential dog or hybrid samples is addressed with a separate 93-SNP wolf-dog panel (Harmoinen et al. 20..., Data is genotyped with microfluidic 96.96 Dynamic Arrays™ (Standard BioTools Inc, formerly Fluidigm) using the 96 SNP panel developed in the corresponding article., # Development of a cost-effective, multifunctional SNP panel and analysis workflow for wolf monitoring in Finland

Dataset DOI: [10.5061/dryad.80gb5mm1t](10.5061/dryad.80gb5mm1t)

## Description of the data and file structure

The data were genotyped using microfluidic 96.96 Dynamic Arrays™ with a 96-SNP panel developed in the corresponding article. Some files also include data genotyped with the Illumina CanineHD Whole-Genome BeadChip microarray (Illumina, Inc., San Diego, California, USA), from which the corresponding 96 SNPs were extracted.

### Files and variables

#### File: Subset_MonitoringData.csv

**Description:** Tab-delimited file including all unique individuals from the subset of monitoring data (supplementary table 5). Output from Allele match software. Missing values indicated by -9.

##### Variables:

Sample name in the first column, and then allele a and b for each loci

#### File: Pedigree_SNP_data.ped

**Description:** PLINK PED file of inbred wolf packs comprising 81...,