Data from: Identification of the submergence tolerance QTL come quick drowning1 (CQD1) in Arabidopsis thaliana

Global climate change is predicted to increase water precipitation fluctuations and lead to localized prolonged floods in agricultural fields and natural plant communities. Thus, understanding the genetic basis of submergence tolerance is crucial in order to improve plant survival under these conditions. In this study, we performed a quantitative trait locus (QTL) analysis in Arabidopsis to identify novel candidate genes for increased submergence tolerance by using Kas-1 and Col (gl1) parental accessions and their derived recombinant inbred lines (RILs). We measured survival after submergence in dark for a 13-day period and used median lethal time, LT50 values for the QTL analysis. A major QTL, the Come Quick, Drowning (CQD1) locus, was detected in two independent experiments on the lower arm of chromosome 5 involved in higher submergence tolerance in the parental accession Kas-1. For fine-mapping, we then constructed near isogenic lines by backcrossing the CQD1 QTL region. We also analyzed QTL regions related to size, leaf number, flowering or survival in darkness and none of the QTL related to these traits overlapped with CQD1. The submergence tolerance QTL, CQD1, region detected in this study includes genes that have potential to be novel candidates effecting submergence tolerance such as TREHALOSE-6-PHOSPHATE PHOSPHATASE and RESPIRATORY BURST OXIDASE PROTEIN D. Gene expression and functional analysis for these genes would reveal the significance of these novel candidates and provide new perspectives for understanding genetic basis of submergence tolerance.据预测，全球气候变化将加剧降水波动，并导致农田与自然植物群落出现局地性持续洪涝灾害。因此，解析植物淹水耐受性的遗传基础，对提升该胁迫条件下植株的存活率至关重要。 本研究以拟南芥（Arabidopsis）为材料，利用亲本生态型Kas-1与Col (gl1)及其衍生的重组自交系（recombinant inbred lines, RILs），开展数量性状位点（quantitative trait locus, QTL）分析，以筛选可提升植物淹水耐受性的新型候选基因。实验中，我们测定了植株在黑暗环境下经历13天淹水后的存活率，并以半数致死时间（median lethal time, LT50）作为QTL分析的量化指标。 在两项独立实验中，我们于5号染色体长臂区域检测到一个主效QTL——CQD1位点（全称Come Quick, Drowning），该位点可赋予亲本生态型Kas-1更高的淹水耐受性。为实现该位点的精细定位，我们通过回交导入CQD1 QTL区域，构建了近等基因系。此外，我们还分析了与植株大小、叶片数、开花时间及黑暗环境下存活率相关的QTL区域，结果显示上述性状相关的QTL均未与CQD1位点发生重叠。 本研究检测到的CQD1淹水耐受性QTL区域内，包含若干有望作为淹水耐受性新型调控候选基因的序列，例如海藻糖-6-磷酸磷酸酶（TREHALOSE-6-PHOSPHATE PHOSPHATASE）与呼吸爆发氧化酶蛋白D（RESPIRATORY BURST OXIDASE PROTEIN D）。对上述候选基因开展基因表达与功能分析，将有助于阐明这些新型候选基因的调控意义，并为解析植物淹水耐受性的遗传基础提供全新视角。