Data for: Altered Circadian Rhythm, Sleep, and Rhodopsin 7-Dependent Shade Preference During Diapause in Drosophila Melanogaster

To survive adverse environments, many animals enter a dormant state such as hibernation, dauer, or diapause. Various Drosophila species undergo adult reproductive diapause in response to cool temperatures and/or short day-length. While it is known that flies are less active during diapause, an in-depth understanding of diapause effects on circadian rhythms and sleep is lacking. Here we show that, in diapause-inducing conditions, Drosophila melanogaster exhibit altered circadian activity profiles, including a severely reduced morning activity peak and an advanced evening activity peak. Consequently, the flies have a single activity peak at a time similar to when non-diapausing flies have a siesta. Temperatures ≤15 °C, rather than short day-length, primarily drive the behavior. At cool temperatures, flies also rapidly enter a deep sleep state that lacks the sleep cycles of flies at higher temperatures and requires particularly high levels of stimulation for arousal. Furthermore, we show t..., Refer to the linked manuscript (\"Altered circadian rhythm, sleep, and rhodopsin 7-dependent shade preference during diapause in Drosophila melanogaster\"., , # Data for: Altered Circadian Rhythm, Sleep, and Rhodopsin 7-Dependent Shade Preference During Diapause in Drosophila Melanogaster

[https://doi.org/10.5061/dryad.0p2ngf28n](https://doi.org/10.5061/dryad.0p2ngf28n)

Enclosed are the source data for the article \"Altered circadian rhythm, sleep, and rhodopsin 7-dependent shade preference during diapause in Drosophila melanogaster.\"

Each figure has a separate .xlsx file, with the source data for each panel provided on a separate sheet (unless indicated otherwise). Due to the large array size in Figure 3d, we have included these data in a separate MATLAB file, \"Figure_3d_StimSpeedTable.mat.\" 

Image data associated with Figure 6 and SI Figure 4 are saved as .czi files, which are binned in zip files.

The zip file names containing raw image data and their corresponding figures are as follows:

* Figure 6a: Figure_6a.zip
* Figure 6c: Figure_6c.zip
* SI Figure 4 c and d: SI_Fig4_C_D.zip

## Code/Software

GitHub repository: [https://github.co...