Concentrations of chlorophyll-alpha combined with different size fractions of microplankton in sea water from the euphotic zone of the Aegean Sea at Station MARCH-1997-GN36199704603MNB05

Samples from 2 l of sea water were size fractionated by filtration through polycarbonate Millipore filters with pores 0.2 µm, 1.2 µm, and 3.0 µm. The filters were kept deep frozen and analyzed according to Holm-Hansen et al. (Holm-Hansen, O., Lorenzen, C.J., Holmes, R.W., Strickland, J.D.H. 1965. Fluorometric determination of chlorophyll. Journal du Conseil Permanent International Pour L'Exploration de la Mer 30, 3-15).取2升海水样本，经孔径为0.2μm、1.2μm及3.0μm的密理博（Millipore）聚碳酸酯滤膜过滤以完成粒径分级分离。将滤膜深冻保存后，依照Holm-Hansen等人提出的分析方法（Holm-Hansen, O., Lorenzen, C.J., Holmes, R.W., Strickland, J.D.H. 1965. 叶绿素的荧光测定法. 《国际海洋考察理事会期刊》30卷，第3-15页）进行分析。