Probabilistic modeling of personalized drug combinations from integrated chemical screen and molecular data in sarcoma

DNA and RNA were isolated from snap frozen primary tumor tissue (human and mouse), and DNA was isolated from matched normal tissue (region matched non-tumor tissue for human, tail section for mouse). A mouse leg was also injected with cardiotoxin to induce tissue damage was taken following sacrifice and was snap frozen, then processed for RNA isolation to serve as a mouse tissue-normal for RNA sequencing. All sequencing was performed at the OHSU MPSSR core. Overall design: Tumor and normal tissues were unpurturbed from procurement to snap freezing, so there are no experimental conditions for any of the samples submitted. A mouse limb was treated with cardiotoxin to induce physical damage and stimulate the wound response to create a similar environment to tissue bearing alveolar rhabdomyosarcoma.