Comparison of quality of sequencing of the BLESS samples using Illumina default protocol (template of length 4) and our Long Template Protocol (template of length 20).

Columns: Sample name, template generation protocol used, % of low diversity sample, % of diverse control, cluster density on the flowcell (expressed as percentage of optimal cluster density), number and percentage of reads with Phred score >30, percentage of reads with intact BLESS barcode among reads with bases with Phred scores >30 normalized for the percentage of the BLESS sample (i.e. if 10% of the sample is BLESS and 10% of reads among Q30 reads are barcoded, that percentage would be normalized to 100%), number of reads with intact BLESS barcode mapped to the human genome with 0 errors. Note that samples A4, LT20_1 and LT20_2 are technical replicates and there is 70-fold increase in number of reads with intact BLESS barcode after switching to our Long Template Protocol.Comparison of quality of sequencing of the BLESS samples using Illumina default protocol (template of length 4) and our Long Template Protocol (template of length 20).