Differentiation of entire complement of spinal sensory interneurons from mouse embryonic stem cells (mESCs) [10X]

By utilizing single-cell analysis of neurons that are produced at day 9 of mESC differentiation period (day 9) under the developmentally relevent signals- Retinoic acid and BMP4, we identified that our protocol can generate all major sensory neuron subtypes that are required for relaying sensation like pain, itch, heat and proprioception in the spinal cord. This analysis also identified functional features in each neuronal subtypes, confirming the bonafide status of the in vitro derived sensory neurons. Overall design: Two 10X genomics chromium samples from mouse ESC differentiations under RA and RA+BMP4 conditions.