Studies of Vietnamese pteridophyte flora 3

Based on recent collections, we reported three new national records of ferns for Vietnam: Haplopteris yakushimensis, Lindsaea kohkongensis, and Pteris pseudowulaiensis. We conducted phylogenetic analyses to investigate the placements of Lindsaea kohkongensis and Leptochilus poilanei, each based on three plastid DNA markers. Our results revealed that Lindsaea kohkongensis is sister to L. ensifolia, while Leptochilus poilanei is embedded within L. cantoniensis. We discussed these results in the context of systematics. Lastly, we reported chromosome numbers for 20 fern species in Vietnam. For seven of these species, including Gymnosphaera salletii, Lepisorus spicatus, Leptochilus poilanei, Pteridrys costularis, Pteris latipinna, Pyrrosia eberhardtii, and Tectaria setulosa, these counts were recorded for the first time. Additionally, three new cytotypes were identified for Diplazium doederleinii, Pteris esquirolii, and Tectaria harlandii., , , # Studies of Vietnamese Pteridophyte Flora 3

[https://doi.org/10.5061/dryad.4j0zpc8mz](https://doi.org/10.5061/dryad.4j0zpc8mz)

## Description of the data and file structure

The two concatenated alignments (in nexus format) and the resulting phylogenetic trees generated by maximum likelihood (ML) analyses using IQTREE.

### Files and variables

#### File: Lindsaea\_3\_reigons\_concatenated.nex

**Description:** Three chloroplast markers including *matK*, *trnH*-*psbA*, and *trnL*-*F.*

#### File: Leptochilus\_3\_regions\_concatenated.nex

**Description:** Three chloroplast markers including *rbcL*, *rps4-trnS*, and *trnL-F.*

#### File: Leptochilus\_ML\_tree.treefile

**Description:** ML phylogeny generated by IQTREE.

#### File: Lindsaea\_ML\_tree.treefile

**Description:** ML phylogeny generated by IQTREE.