Studies of Vietnamese pteridophyte flora 3

Based on recent collections, we reported three new national records of ferns for Vietnam: Haplopteris yakushimensis, Lindsaea kohkongensis, and Pteris pseudowulaiensis. We conducted phylogenetic analyses to investigate the placements of Lindsaea kohkongensis and Leptochilus poilanei, each based on three plastid DNA markers. Our results revealed that Lindsaea kohkongensis is sister to L. ensifolia, while Leptochilus poilanei is embedded within L. cantoniensis. We discussed these results in the context of systematics. Lastly, we reported chromosome numbers for 20 fern species in Vietnam. For seven of these species, including Gymnosphaera salletii, Lepisorus spicatus, Leptochilus poilanei, Pteridrys costularis, Pteris latipinna, Pyrrosia eberhardtii, and Tectaria setulosa, these counts were recorded for the first time. Additionally, three new cytotypes were identified for Diplazium doederleinii, Pteris esquirolii, and Tectaria harlandii., , , # Studies of Vietnamese Pteridophyte Flora 3 [https://doi.org/10.5061/dryad.4j0zpc8mz](https://doi.org/10.5061/dryad.4j0zpc8mz) ## Description of the data and file structure The two concatenated alignments (in nexus format) and the resulting phylogenetic trees generated by maximum likelihood (ML) analyses using IQTREE. ### Files and variables #### File: Lindsaea\_3\_reigons\_concatenated.nex **Description:** Three chloroplast markers including *matK*, *trnH*-*psbA*, and *trnL*-*F.* #### File: Leptochilus\_3\_regions\_concatenated.nex **Description:** Three chloroplast markers including *rbcL*, *rps4-trnS*, and *trnL-F.* #### File: Leptochilus\_ML\_tree.treefile **Description:** ML phylogeny generated by IQTREE. #### File: Lindsaea\_ML\_tree.treefile **Description:** ML phylogeny generated by IQTREE.基于近期采集的标本，我们报道了越南蕨类植物的3个国家级新记录种：*Haplopteris yakushimensis*、*Lindsaea kohkongensis*及*Pteris pseudowulaiensis*。我们基于3个质体DNA分子标记，分别对*Lindsaea kohkongensis*和*Leptochilus poilanei*的系统发育位置开展了分析。结果显示，*Lindsaea kohkongensis*与*L. ensifolia*互为姊妹群，而*Leptochilus poilanei*嵌套于*L. cantoniensis*类群中。我们结合分类学背景对上述结果进行了讨论。最后，我们报道了越南20种蕨类植物的染色体数目，其中7个物种的染色体数目为首次记录，包括*Gymnosphaera salletii*、*Lepisorus spicatus*、*Leptochilus poilanei*、*Pteridrys costularis*、*Pteris latipinna*、*Pyrrosia eberhardtii*及*Tectaria setulosa*。此外，我们还为*Diplazium doederleinii*、*Pteris esquirolii*及*Tectaria harlandii*鉴定出3个新的细胞型。 # 《越南蕨类植物区系研究3》 [https://doi.org/10.5061/dryad.4j0zpc8mz](https://doi.org/10.5061/dryad.4j0zpc8mz) ## 数据与文件结构说明 本数据集包含两份拼接后的序列比对文件（Nexus格式），以及使用IQTREE通过最大似然法（maximum likelihood, ML）分析得到的系统发育树文件。 ### 文件与变量说明 #### 文件：Lindsaea_3_reigons_concatenated.nex **描述：** 包含3个叶绿体分子标记：*matK*、*trnH*-*psbA*及*trnL*-*F*。 #### 文件：Leptochilus_3_regions_concatenated.nex **描述：** 包含3个叶绿体分子标记：*rbcL*、*rps4-trnS*及*trnL-F*。 #### 文件：Leptochilus_ML_tree.treefile **描述：** 使用IQTREE构建的最大似然法系统发育树。 #### 文件：Lindsaea_ML_tree.treefile **描述：** 使用IQTREE构建的最大似然法系统发育树。