Milk metabolome reveals variations on enteric methane emissions from dairy cows fed a specific inhibitor of the methanogenesis pathway

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The objective of this work is to explore changes in milk metabolic profiles associated with the reduction of methane emissions. In this study we performed, untargeted NMR and LC-MS metabolomics analysis, targeted LC-MS and GC-FID analysis. We identified 38 discriminant metabolites which affected 10 metabolic pathways including methane metabolism.