Disfunction of arachidonic acid metabolism in vascular smooth muscle cells contributes to restenosis

We applied the transcriptome profiling (RNA-seq) for high-throughput profiling of genes changes in the phenotypic switch of VSMCs. Rat primary VSMCs were divided into 3 groups, control, PDGF-BB, PDGF-BB+PTUPB,and mRNA sequence were performed. We found that Cell cycle related genes and cellular senescence related genes were significantly upregulated by PDGF-BB and significantly reversed by PTUPB. Subsequently, we deleted PTTG1 as a key gene for PTUPB to reverse phenotypic switching in VSMCs. Our study provided the transcription changes by RNA-seq in VSMC phenotypic switch, and found that PTUPB played a crucial role in correcting the dysregulation of sEH/COX-2 derived ARA metabolism in VSMC phenotypic switch In order to understand how PTUPB reverses the phenotypic transition of PDGF-BB-induced VSMCs, we divided rat primary VSMCs into control, PDGF-BB (20 ng/mL) stimulation for 24 h, and PTUPB pretreatment (0.5 μM,2h) followed by PDGF-BB stimulation for 24 h. We performed NGS RNAseq on these groups.Each group included three VSMCs.