DhQS charged the shikimate pathway pathway in Thermus thermophilus HB27

Temperature stress is a survival challenge that biological cells may encounter at any time. To sustain normal metabolic activities, organisms have gradually evolved strategies to respond promptly to temperature changes. The two-component system, typically involved in regulating the transcription of relevant genes in response to environmental stimuli, consists of a histidine kinase and a response regulator. This system mediates environmental signal transduction in microbial cells. This study identified DhqSR as a novel temperature-sensing two-component system in Thermus thermophilus HB27. It regulates the expression of type II 3-dehydroquinate dehydratase (DHQase) in the aromatic amino acid biosynthesis pathway, indirectly controlling aromatic amino acid synthesis and thereby altering cellular thermal adaptation capabilities. Overall design: ChlP-seq for DhqSR system regulatored genes which work for temperature stress response. The samples were collected for 65°C cultured HB27 cells.