GBS data of seven Brassica cultivars from Pakistan

The genotyping-by-sequencing (GBS) data was generated on seven Brassica cultivars from Pakistan as a purpose of varietal registration. After strict quality-control, only 501 high-quality SNP markers were retained with 0% missing data. These marker sites can be used for varietal identification and diversity analysis. Methods The DNA was extracted using CTAB method, and the sequencing library was prepared following standard guidelines of two restriction enzymes based strategy. The sequencing libraries were sequenced on T7 MGI-Seq platform. The raw sequencing reads were aligned to the Reference genome sequence of Brassica napus, and the SNPs were called using GATK variant calling pipeline. The vcf file generated is being uploaded which include 501 SNPs with 100% data availability.