RNA-array in DOT1L knockout and wild type CD8+ T cells

Disruptor of telomeric silencing 1-like (DOT1L) is the specific and sole methyltransferase for H3K79. However, the role of Dot1l in T cells is not well determined. Here, used CD4-Cre/Dot1lflox/flox mice to specifically delete DOT1L in T cells. The genes characteristics were determined by RNA array and compared to wild type cells. We used microarrays to detail the global gene expression in Dot1l knockout and wild type CD8+ T cells. Dot1l knockout or wild type CD8+ T cells were separated by the EasySep™ Mouse CD8+ T Cell Isolation Kit (STEMCELL Technologies Inc., Cambridge, MA, USA) in the peripheral and spleen of mice. The RNA was isolated by using Direct-zolTM RNA miniprep Plus kit (ZOMO research).