Hfq is a bacterial Sm-like protein which serves as a post-transcriptional regulator of global gene expression. It has been shown to be involved in a wide range of cellular processes and pathways. To c
Total RNA from Brucella abortus 2308 will be extracted at early, mid and late log as well as stationary growth phase in vitro.This will be compared to the in vivo transcriptional signals.
Transcriptional profile compairing infected human macrophages at 2 and 4 h p.i. with virulent strain Brucella melitensis BM16M vs wildtype BM133 , BM16M vs mutant LVM31, BM133 vs 16M. Overall design: