Survival limits of yeasts and other extremophilic microorganisms with relevance for planetary protection of the icy moons

Investigating the survival limits of extremophilic microorganisms exposed to simulated space conditions can shed light on the ability of terrestrial microorganisms to survive and propagate on other planetary bodies. Although microbes can be found in all environmental niches on Earth, this study focuses mainly on psychrophilic and psychrotolerant microorganisms (prokaryotes and eukaryotes) which have been isolated from locations of interest such as icy moon analogue environments and cleanrooms, and which might be of concern for forward planetary protection. Our research aimed to reproduce conditions for microorganisms on spacecraft travelling to the outer solar system, which could contaminate the icy moon’s subsurface oceans. The microorganisms were grown under oligotrophic conditions in minimal media supplemented with only a single carbon source and exposed to extreme conditions, in terms of temperature and radiation, as they occur during space travel to the outer solar system. Our resu..., Methods included growth of microorganisms in minimal media, exposure to desiccation, polychromatic UV, UV-C and X-ray radiation., , # Data from: Survival limits of yeasts and other extremophilic microorganisms with relevance for planetary protection of the icy moons

Dataset DOI: [10.5061/dryad.70rxwdc7r](10.5061/dryad.70rxwdc7r)

## Description of the data and file structure

Data from: Survival of extremophilic microorganisms to icy moon simulated conditions

**Description of the data and file structure**

The data reported here was obtained by determining the relative survival (N/N0) of the microorganisms evaluated in our study exposed to icy moon conditions. These include the exposure to freeze and thaw cycles, to freezing at -80°C without cryoprotectants, the exposure to polychromatic UV radiation, UV-C radiation and X-ray radiation. The relative survival was calculated by dividing the number of microbial colony forming units (CFU) post exposure (N) by the CFU of the control condition (N0) un-exposed to the icy moon conditions. 

### Files and variables

#### File: Relative_survival_to_exposure.zip

**Descripti...,