Proteomic analysis of extracellular vesicles from inflammatory fibroblasts in colorectal cancer lung metastasis

Pulmonary metastasis is a major cause of mortality in colorectal cancer (CRC), and the tumor microenvironment plays a critical role in metastatic progression. In particular, inflammatory fibroblasts have been implicated in shaping the metastatic niche; however, the molecular characteristics of their extracellular vesicles (EVs) remain incompletely understood. In this study, we performed LC-MS/MS-based proteomic analysis of EVs derived from IL-1β–stimulated MRC-5 lung fibroblasts. EVs were isolated from conditioned media of MRC-5 cells with or without IL-1β treatment and subjected to comparative proteomic profiling. This dataset provides a resource for characterizing EV-associated protein alterations in inflammatory fibroblasts and supports further investigation of their potential roles in tumor-related processes in colorectal cancer lung metastasis.