Data from: Secondary structure models of 18S and 28S rRNAs of the true bugs based on complete rDNA sequences of Eurydema maracandica Oshanin, 1871 (Heteroptera: Pentatomidae)

The sequences of 18S and 28S rDNAs have been used as molecular markers to resolve phylogenetic relationships of Heteroptera for two decades. The complete sequences of 18S rDNAs have been used in many studies, while in most studies only partial sequences of 28S rDNAs have been used due to technical difficulties of amplifying the complete lengths. In this study, we amplified the complete 18S and 28S rDNA sequences of Eurydema maracandica Oshanin, 1871, and reconstructed the secondary structure models of the corresponding rRNAs. In addition, and more importantly, all of the length variable regions of 18S rRNA were compared among 37 families of Heteroptera based on 140 sequences, and the D3 region of 28S rRNA was compared among 51 families based on 84 sequences. It was found that 8 length variable regions could potentially serve as molecular synapomorphies for some monophyletic groups. Therefore discoveries of more molecular synapomorphies for specific clades can be anticipated from amplification of complete 18S and 28S rDNAs of more representatives of Heteroptera.近二十年来，18S核糖体DNA（18S rDNA）与28S核糖体DNA（28S rDNA）序列已作为分子标记，用于解析异翅亚目（Heteroptera）的系统发育关系。诸多研究均采用了18S rDNA的全长序列，但由于扩增28S rDNA全长序列存在技术难点，多数研究仅使用其部分序列。本研究扩增了Eurydema maracandica Oshanin, 1871的18S和28S rDNA全长序列，并构建了对应rRNA的二级结构模型。此外更为关键的是，本研究基于140条序列，对异翅亚目37个科的18S rRNA全部长度可变区开展了比较分析；同时基于84条序列，对51个科的28S rRNA D3区域进行了比对。研究发现，8个长度可变区有望作为部分单系群（monophyletic groups）的分子共有衍征（molecular synapomorphies）。因此，通过扩增更多异翅亚目类群代表的18S和28S rDNA全长序列，有望为特定支系（clades）发掘更多分子共有衍征。