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Amplicon sequencing of CRISPR/Cas9 edited Lbx-A gene in lampreys.

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https://www.ncbi.nlm.nih.gov/sra/DRP006419
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资源简介:
To clarify which of the lamprey larval muscles require function of Lbx-A during development, we disrupted LjLbx-A locus during embryogenesis, using CRISPR/Cas9-mediated gene editing. In order to confirm the effect of gene editing, amplicon library was constructed using a multiplexed method, and DNA sequencing was performed by Illumina MiSeq.
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2020-10-20
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