SRE4 Effects of hydrocarbons on Antarctic Marine Sediment Ecosystems - SRE4_Meiofauna

Sediment Recruitment Experiment 4 (SRE4) was a large, long term (5 year) field experiment run at Casey Station (from 2001 to 2006) testing the effects of 4 different hydrocarbons on marine sediment ecosystems. Four different types of hydrocarbons were individually mixed with defaunated marine sediments and deployed in trays on the seabed at O'Brien Bay-1. Trays were collected after deployment periods of 5 weeks, 56 weeks (one year), 62 weeks, 2 years and 5 years. In addition there was a bioturbation treatment using the burrowing urchin Abatus (at 56 weeks only). Samples were collected from 4 replicate trays of each treatment at each sampling time. Analyses were done of sediment hydrocarbon chemistry, microbial communities, meiofaunal communities, macrofaunal communities and diatom communities. The hydrocarbon treatments were: a synthetic Mobil lubricating oil; the same Mobil lubricating oil after 125 hours use in a vehicle engine; a Fuchs synthetic lubricating oil marketed as highly biodegradable; and Special Antarctic Blend diesel fuel (SAB). A control uncontaminated sediment treatment was used for comparison.A randomised block design was used with a tray of each treatment in 24 blocks (5 trays per block). Four blocks were sampled at each time (4 trays of each treatment).MeiofaunaTwo replicate cores were taken from each tray at each time.Copepods were identified to family. Nematodes were identified to genus.Data is from one, two and five years of the experiment. At one year there is only one replicate per tray for copepod data (a total of 4 replicates per treatment).沉积物补充实验4（Sediment Recruitment Experiment 4，SRE4）是一项于2001年至2006年在凯西站（Casey Station）开展的大型长期野外实验，实验周期长达5年，旨在探究4种不同烃类物质对海洋沉积物生态系统的影响。研究人员将4类不同烃类分别与经除动物处理的海洋沉积物（defaunated marine sediments）混合，随后将混合物放置于奥布莱恩湾1号（O'Brien Bay-1）海床的托盘内。实验托盘分别在部署5周、56周（即1年）、62周、2年及5年后进行回收。此外还设置了一项生物扰动处理，仅在56周时使用穴居海胆Abatus开展相关实验。每个处理组在每个采样时间点均设置4个重复托盘以采集样本，后续分析涵盖沉积物烃类化学组成、微生物群落、小型底栖生物（Meiofauna）群落、大型底栖生物群落及硅藻群落。本次实验的烃类处理组包括：美孚（Mobil）合成润滑油；经车辆发动机使用125小时后的同款美孚润滑油；以高生物降解性为宣传卖点的福斯（Fuchs）合成润滑油；以及特种南极混合柴油（Special Antarctic Blend diesel fuel，SAB）。同时设置未受污染的沉积物对照组用于对照分析。实验采用随机区组设计，每个区组内包含各处理组对应的1个托盘，共设置24个区组（每个区组含5个托盘）。每个采样时间点采集4个区组的样本，即每个处理组对应4个重复托盘。小型底栖生物采样环节：每个托盘在每个采样时间点采集2个重复岩心。其中桡足类（Copepods）鉴定至科水平，线虫类（Nematodes）鉴定至属水平。本数据集取自实验开展1年、2年及5年的样本数据，其中1年采样节点的桡足类数据仅包含每个托盘的1个重复样本，即每个处理组总计4个重复样本。