Under Normoxia Simvastatin Shifts Lipid Metabolism of B16.F10 Melanoma Cells to Support Their Aggressiveness

Supplementary data to paper: "Under Normoxia Simvastatin Shifts Lipid Metabolism of B16.F10 Melanoma Cells to Support Their Aggressiveness" by Negrea et al., 2024. These data represent: 1) the Supplementary Table corresponding to all the mRNA identified by RNA-seq in B16.F10 melanoma cells treated with lipophilic statin simvastatin (SIM), compared to the untreated cells. Our data revealed a panel of 1512 out of 15905 transcripts with at least 1.5 FR in expression after SIM treatment, with 816 transcripts upregulated, and 696 downregulated compared to the control group; 2) Figure 1. Validation of primer specificity by agarose electrophoresis of PCR products; 3) Figure 2. Relative expression of genes involved in metabolic pathways assessed by RT-qPCR; 4) Supplementary Table 2. GO and KEGG analysis results; 5) Supplementary Table 3. PPI data; 6) Supplementary Table 4. DEGs related to Aggresiveness.本数据集为Negrea等人2024年发表的学术论文《常氧条件下辛伐他汀重塑B16.F10黑色素瘤细胞脂质代谢以促进其侵袭性》的补充数据。本数据集包含以下内容：1) 对应经亲脂性他汀类药物辛伐他汀（SIM）处理的B16.F10黑色素瘤细胞与未处理对照组细胞的RNA测序（RNA-seq）鉴定所得全部mRNA的补充表格。本研究数据显示，在15905个转录本中，经辛伐他汀处理后有1512个转录本的表达量存在至少1.5倍的变化（FR），其中上调转录本816个，下调转录本696个；2) 图1：通过琼脂糖凝胶电泳验证PCR产物的引物特异性；3) 图2：经实时定量聚合酶链反应（RT-qPCR）检测的代谢通路相关基因的相对表达水平；4) 补充表2：基因本体（GO）与京都基因与基因组百科全书（KEGG）分析结果；5) 补充表3：蛋白质相互作用（PPI）数据；6) 补充表4：与侵袭性相关的差异表达基因（DEGs）数据。