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We need to sequence commonly used edited iPSC cell lines in order to gain accurate sequence data of the entire genome (including non-coding regions) including haplotype information to allow the better design of CRISPR reagents and homology constructs for introducing SNPs and other defined genomic changes. For instance the KOLF2-C1 Arid 2 corrected F9 Het Bxb1-Blast-mApple cell line or other cell lines that differentiate into other cell types.

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2025-04-17
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