Gastric single-cell RNA-sequencing of mice with Helicobacter pylori infection and/or KRAS-driven metaplasia

Stomachs from mice +/- H. pylori infection, +/- induction of active KRAS in gastric Mist1-expressing cells, were used for gastric single-cell RNA-sequencing. Samples were obtained six and 12 weeks after infection/KRAS induction. Six samples were frozen, then thawed before sequencing (and in some cases pooled from multiple mice) and two samples were freshly prepared and never frozen before sequencing. Samples were obtained six and 12 weeks after infection/KRAS induction.