RNA-sequencing analysis of colon RNA from mice with compound mutations of XBP1, caspase-8, TNFR1 and MLKL.

To purpose of this study was to assess how intestinal epithelial cell (IEC)-specific ablation of XBP1 or caspase-8 alone or in combination affected mucosal immune homeostastis and inflammation, and the role of TNFR1 and MLKL in the associated phenotypes. First, expression of colonic RNA from mice with combined IEC-specific XBP1 and caspase-8 ablation (Xbp1IEC-KO Casp8IEC-KO) was compared with Casp8IEC-KO, Xbp1IEC-KO, and wild-type mice. In addition, Xbp1IEC-KO Casp8IEC-KO Mlkl-/- and Xbp1IEC-KO Casp8IEC-KO Tnfr1IEC-KO mice were compared with Xbp1IEC-KO Casp8IEC-KO, Casp8IEC-KO, Xbp1IEC-KO as well as wild-type control mice. For each mouse line 5 individual mice were analysed. Overall design: RNAs from the colons of control, Xbp1IEC-KO, Casp8IEC-KO, Xbp1IEC-KO Casp8IEC-KO, Xbp1IEC-KO Casp8IEC-KO, Xbp1IEC-KO Casp8IEC-KO Tnfr1IEC-KO and Xbp1IEC-KO Casp8IEC-KO Mlkl-/- mice were isolated. Then RNA-sequencing-assisted gene expression analysis were performed from these isolated RNAs.