Molecular weight (Mw) and hydrodynamic radius (Rh) of crystallins in αB-R120G mice.

*Proteins from completely opaque 9-month-old homozygous mutant lenses were largely water-insoluble. The estimated molecular weight of the α-crystallin fraction was >10×106. Most of the lens proteins did not go through the column filter (0.2-µm). The concentration of protein that went through the column was very low (0.128 mg/ml). Insufficient soluble protein made it difficult to accurately evaluate the Rh value from viscosity measurements.**Protein concentration of the homozygous lens proteins was too low for an accurate determination of Rh. Data for two independent sets of 4-month-old mutant lenses (group 1 and group 2) demonstrate minor differences between litters.WT, wild-type; Het, heterozygous; Homo, homozygous.