Characterization of the role of SETDB1 in Muscle stem cells during skeletal muscle regeneration [ChIP-seq]

We are characterizing the role of an H3K9 histone methyltransferase. As these proteins have the capacity to modulate chromatin, we investigate the chromatin modulation using H3K9Me3 Chip-seq. Overall design: Muscle Stem Cells were isolated from the conditional mice model Setdb1-cKO and were isolated by Magnetic-activated cell sorting (MACS) and cultured in vitro. We treated the cells with either Ethanol or 4-hydroxytamoxifen in order to induce Setdb1 deletion and after 72 hours of treatment performed the H3K9Me3 Chip-Seq.