Effects of 4-1BB ligation in Ag85B-activated spleen cells of P-mice on cytokine/chemokine production.

Unfractionated spleen cells of P-mice were cultured with Ag85B protein (5 µg/ml) in the presence of IgG2a control Ab or agonist anti-4-1BB mAb (5 µg/ml). Culture supernatants were harvested at 48 h for IL-2, IL-4; at 72 h for IL-6, IL-10, XCL-1, MIP-1β, TGF-β1, TNF-α and at 96 h for IL-17 detection by specific quantitative sandwich ELISA Kits. The results are presented as mean ± SEM in pg/ml for cytokine detection. Pooled data of 3 independent experiments are shown.