A low-cost and customizable alternative for commercial implantable cannula for intracerebral administration in mice

Repository of design files for the HardwareX article titled "A low-cost and customizable alternative for commercial implantable cannula for intracerebral administration in mice". Contributing authors: Rogneda B Kazanskaya, Alexander V Lopachev, Tatiana N Fedorova, Raul R Gainetdinov, Anna B Volnova. Abstract: Stereotaxic intracerebral cannula implantation for neuroactive agent administration is a wide-spread method for chronic experiments requiring bypassing the blood-brain barrier in rodents. However, commercially available cannula are bulky and may interfere with animal movement or lead to their dislodging during grooming. As the number of cannula needed in one experiment, and the accompanying costs can be high, it is in the interest of researchers to produce them on their own. Custom cannula manufacturing also offers the flexibility of different cannula lengths, which is required for agent delivery to various brain structures. In this article we present a protocol for making guide cannula along with the accompanying systems required for injection, which are small, cost-effective, light, easy to make, reusable, and can be made from easily procured materials.本仓库为发表于"HardwareX"期刊的《面向小鼠脑内给药的商用植入式套管的低成本定制化替代方案》一文配套的设计文件仓库。共同作者：罗格涅达·B·卡赞斯卡娅（Rogneda B Kazanskaya）、亚历山大·V·洛帕乔夫（Alexander V Lopachev）、塔蒂亚娜·N·费多罗娃（Tatiana N Fedorova）、劳尔·R·盖涅季诺夫（Raul R Gainetdinov）、安娜·B·沃尔诺娃（Anna B Volnova）。摘要：用于递送神经活性物质的立体定位脑内套管植入术，是啮齿类动物慢性实验中绕过血脑屏障的常用方法。然而，市售套管体积偏大，可能干扰动物活动，或在动物梳理毛发时导致套管脱落。由于单次实验所需套管数量及配套成本往往较高，研究者自行制作套管符合自身研究需求。定制化套管制造还可灵活调整套管长度，这正是将活性物质递送至不同脑区所必需的。本文提出了一种制作引导套管及其配套注射系统的方案，该方案具备体积小巧、成本低廉、重量轻盈、制作简便、可重复使用的优势，且可通过易于获取的材料制成。