RNA sequencing reveals the genetic relationship between TOPPs and its regulatory subunit PP1R3 in Arabidopsis thaliana. RNA sequencing reveals the genetic relationship between TOPPs and its regulatory subunit PP1R3 in Arabidopsis thaliana

We identified a new regulatory subunit PP1R3 of type 1 protein phosphatase in Arabidopsis by immunoprecipitation coupled with liquid chromatography-mass spectrometry.Through RNA sequencing, it was found that there are few differentially expressed genes (DEGs) between pp1r3-2 and topp septuple mutants (topp-7m).Moreover, the DEGs of pp1r3-2 compared to the wild type (Col) have a great overlap with the DEGs of topp-7m compared to Col, and the change trend of gene expression is very similar.At the same time, we also found that pp1r3-2 can partially restore the gene expression changes in the dominant negative mutant topp4-1.And, the DEGs of pp1r3-2 and pp1r3-3 topp4-1 are less than one hundred, which is less than one-tenth of the number of DEGs of pp1r3-2 compared to Col.These results reveal a strong genetic interaction between PP1R3 and TOPPs at the gene expression level. Overall design: 3-week greenhouse-grown wild type Col and mutants pp1r3-2, topp-7m, topp4-1 and pp1r3-2 topp4-1