Water Quality Data (Extensive) from the Taylor Slough, Everglades National Park (FCE LTER), Florida, USA, April 1996 - ongoing

Water quality samples are being collected using ISCO autosamplers at all wetland sites (that is, all sites except TS/Ph-9, 10, and 11). The autosamplers contain 24 1L bottles. Water is sampled by programming the autosamplers to take composite samples once every 3 days. These samples are a composite of four 250mL subsamples drawn every 18 hours (a sampling scheme that captures a dawn, noon, dusk, and midnight sample in every three day composite). The samples are collected every 3-4 weeks and analyzed for total phosphorus (TP), total nitrogen (TN), and salinity. When sites are visited to collect these samples, we also collect a grab sample that is immediately put on ice. A portion of these grab samples is filtered through a Whatman GF/F filter immediately upon return to the lab, and the filtered samples are analyzed for inorganic nutrients such as NO2-, NO3-, NH4+, SRP, and DOC. The unfiltered fraction of these grab samples is analyzed for TP, TN, and TOC. We use these monthly grab samples to generate relationships between TP and SRP, and between TN and NO2- + NO3- + NH4+. Dissolved nutrients are measured using standard rapid flow analyzer (RFA) techniques. TP is analyzed with a modified Solorzano and Sharp (1980) technique. TN is measured with an Antec TN analyzer, TOC and DOC are quantified on a Shimadzu TOC Analyzer, and salinity is measured with an YSI conductivity meter. In addition to the regular water quality monitoring, we use the rain level actuators at all freshwater sites to trigger water sampling after rain events exceed a given threshold of duration and/or intensity. As currently programmed, when the threshold of = 2.5 cm of rain per hour is passed, the autosampler at that site collects a 500mL sample 30 minutes after the threshold has been reached. Rain event samples are collected, treated, and analyzed as all other water quality samples.本研究采用ISCO自动采样器（ISCO autosamplers）对所有湿地监测点位开展水质样品采集，覆盖除TS/Ph-9、10和11号点位外的全部站点。该自动采样器配备24个1升储样瓶，通过编程设置每3天采集一次混合水样：该混合水样由每18小时采集的4份250毫升子样混合而成，此采样方案可在每3天的混合水样中覆盖黎明、正午、黄昏与午夜四个时段的样品。样品每3至4周采集一次，分析指标包括总磷（TP）、总氮（TN）与盐度。在现场采集上述样品的同时，还会同步采集瞬时水样，并即刻置于冰上冷藏保存。返回实验室后，立即使用Whatman GF/F滤膜（Whatman GF/F filter）对部分瞬时水样进行过滤，过滤后的样品用于分析亚硝酸盐氮（NO₂⁻）、硝酸盐氮（NO₃⁻）、氨氮（NH₄⁺）、可溶性活性磷（SRP）以及溶解性有机碳（DOC）等无机营养盐；未经过滤的瞬时水样则用于分析总磷、总氮与总有机碳（TOC）。本研究利用这些月度瞬时水样，建立总磷与可溶性活性磷之间、总氮与亚硝酸盐氮+硝酸盐氮+氨氮之间的相关性关系。溶解性营养盐采用标准快速流动分析仪（RFA）技术进行测定。总磷采用改良的Solorzano与Sharp（1980）方法开展分析；总氮使用Antec总氮分析仪完成测定；总有机碳与溶解性有机碳通过岛津TOC分析仪（Shimadzu TOC Analyzer）定量；盐度则采用YSI电导率仪（YSI conductivity meter）测定。除常规水质监测外，本研究还为所有淡水监测点位配置了雨量触发装置，当降雨事件的持续时长和/或强度达到设定阈值时，自动启动水样采集流程。按照当前的编程设置，当每小时降雨量达到2.5厘米的阈值时，对应点位的自动采样器将在阈值触发后30分钟采集500毫升水样。降雨事件采集的样品，其处理与分析流程与其他常规水质样品完全一致。