Sequencing of Bordetella bronchiseptica KU1201. Bordetella bronchiseptica strain:KU1201

Bordetella bronchiseptica KU1201 was isolated from soil sample through enrichment culture on phenylmalonate as a sole carbon source. We identified that this strain harbors a novel unique enzyme that shows decarboxylation activities to arylmalonates, materials unfound in natural environment. The enzyme (AMDase) catalyzes an asymmetric decarboxylation of pro-chiral arylmalonates to produce optically pure (R)-arylcarboxylates without any cofactor. However, physiologically true substrate(s) catalyzed by AMDase and the metabolic pathway that this enzyme mediates are still uncovered. In this project, we try to sequencing whole genome of Bordetella bronchiseptica KU1201 by Illumina GAIIx.